One-step purification of chitinase from Serratia marcescens NK1, a soil isolate
نویسندگان
چکیده
منابع مشابه
Optimization of Process Parameters for Chitinase Production by a Marine Isolate of Serratia marcescens
Chitinases play an important role in the decomposition of chitin and results in the utilization of chitin as a renewable resource. A total of 18 chitinolytic bacteria were isolated from the sand sample. Following primary and secondary screening in colloidal chitin medium, strain CBC-5 demonstrated the highest chitinolytic activity and was selected for further study. It was later identified as S...
متن کاملSerratia marcescens B4A Chitinase Product Optimization Using Taguchi Approach
Chitinase production by newly isolated Serratia marcescens B4A was optimized following Taguchi’sarray methods. Twenty-three bacterial isolates were screened from shrimp culture ponds in the South ofIran. A chitinase-producing bacterium was isolated based on it’s ability to utilize chitin as the sole carbonsource. The isolate designated as B4A, was identified as Serratia marces...
متن کاملCloning of a Serratia marcescens Gene Encoding Chitinase.
Serratia marcescens, a chitinase-producing microorganism, was shown to produce five unique chitinolytic proteins with subunit molecular masses of 21, 36, 48, 52, and 57 kilodaltons. A cosmid library of S. marcescens DNA was constructed in the broad-host-range cosmid pLAFR1 and screened in Escherichia coli for clones capable of degrading chitin. A total of four independent clones (22- to 27-kilo...
متن کاملPurification and partial characterization of a bacteriocin from Serratia marcescens.
Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m urea. In the absence of these agents, the bacteriocin associates into aggregate...
متن کاملGenetic analysis of the chitinase system of Serratia marcescens 2170.
To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the presence of chitin were analyzed. Four chitinases, A, B, C1, and C2, among other p...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2001
ISSN: 1364-5072,1365-2672
DOI: 10.1046/j.1365-2672.2001.01308.x